Pools of pre-intended primers can help generate comprehensive expression profiles, Specifically the place assay sensitivity is critically essential.
Unlike oligo(dT) primers, these primers usually are not template unique and they are suitable for DNA synthesis making use of Klenow fragments with DNA templates or for cDNA synthesis applying reverse transcriptase with mRNA templates.
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Soon after the introduction of your .222 Remington experiments started to make an analogous cartridge for military services use. The benefits had been noticeable as a soldier naturally can have numerous extra rounds of the Substantially smaller and lighter cartridge...
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In distinction to random primers, oligo (dT) primers are usually not suitable for degraded samples but are outstanding for developing cDNA libraries and encouraged for use with reverse transcriptases (RTs) as Superscript IV RT.
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